OCT Compound Tissue Freezing

OCT Compound Tissue Freezing is a technique used to freeze tissues with a high degree of precision. This method is most effective for cryosections of heart tissue from zebrafish, Sprague Dawley rats, and C57BL/6 mice. In addition, OCT can be used for sciatic nerve cryosections. Listed below are some of the most common applications for OCT.

Hearts from zebrafish, newts, or Sprague Dawley rats

A study in which the hearts of adult zebrafish, newts, and Sprague Dawley rats were treated with nocodazole and serum, then iced, was published. The resulting hearts were stained with DAPI and centrosomes were identified using yellow arrowheads. These experiments were performed to determine if PCNT splice isoforms were preserved in the heart.

The study was conducted on adult zebrafish, newt, and rat hearts and was performed to determine if the heart tissue can be preserved. A Leica CM 3000 cryostat was used to section the hearts and isolate the organs. The sections were stained with hematoxylin-FITC and DAPI to determine if the heart tissue was preserved.

PCNT immunostaining of cardiomyocytes using a plasmid containing human or rat mRNAs showed that the cells had been transfected with the MTOC promoter gene. This gene was expressed in the hearts of zebrafish, newts, and Sprague Dawley rats.

The findings were consistent. The average maximum rate of the hearts of the three species was similar in all three experiments. However, in the zebrafish experiments, higher rates were observed with electrical stimulation. In the newt and Sprague Dawley rats, the maximum rate of cardiac beating was greater than in the zebrafish heart.

The results showed a good correlation between in vivo measurements and histological staining. The 3D multi-slice model showed a promising match with the postmortem results. This method is more comprehensive than conventional 2D tomographic analysis. In addition, it also provides a better view of cardiac perfusion.

OCT Compound is a water-soluble blend of glycols and resins developed specifically for cryostat sections. The compound leaves no residue on slides during staining, eliminating the unwanted background staining that can occur with conventional techniques. OCT Compound is recommended for use with chromogenic immunohistochemistry. Furthermore, it does not cause microtome knives to dull.

OCT Compound Tissue Freezing of human hearts is an important way to study heart disease. Genetically modified mice have been used as experimental models for human heart function and disease. This method allows scientists to study the function of human heart tissue without compromising its intrinsic differences. It is a promising approach for studying human heart disease and improving drug development.

Hearts from C57BL/6 mice

To preserve the heart of a C57BL/6 mouse, it is necessary to prepare a cryomold for the preservation of tissue. The tissue is pressed into the mold with the rostral region toward the top. To minimize the risk of tissue cracking, the OCT compound must be applied sparingly to the mold. Also, large pieces of tissue should not be crowded in the mold.

The OCT compound is used for embedding fresh tissues in preparation for cryosectioning. This compound contains water-soluble glycols and resins to form a solid matrix for tissue specimens. The OCT compound prevents background staining and results in fast freezing. The compound is available in four-oz squeeze bottles and 12 per case. To prevent freezer burn, store the OCT blocks in a -80 degC deep freezer.

The use of skinned cardiac muscle strips is beneficial to researchers as it allows them to collect high-quality skinned cardiac muscle. This technique also preserves the integrity of myofilament proteins and facilitates resource sharing. The technique also preserves the integrity of muscle strips, allowing for comprehensive mechanical studies, coupled characterization of myofilament proteins, and post-translational modifications.

The OCT technique is also compatible with fresh tissue. Precast gelatin-based molds are used for cryosectioning. Mice brain tissue is frozen using precast gelatin-based molds. The gelatin-based molds were mounted onto a glass slide. The tissue was sectioned at 25 mm. The precast molds were then discarded after sectioning. The MALDI-MSI analysis of the images revealed that there was no contamination with OCT polymeric material.

The heart of a C57BL/6 mouse may also be affected. OCT compound tissue freezing has been shown to lead to heart thinning, and in a number of other ways. Hearts from C57BL/6 mice may be prone to clotting due to OCT compound tissue freezing. Moreover, the OCT compound can cause a heart to fail to function due to the presence of a particular protein, such as OCT.

The anti-b-galactosidase antibody from IBL produced indistinguishable vascular and junction-associated staining in the WT and claudin-12lacZ/lacZ mouse. However, the anti-b-galactosidase antibody from Invitrogen did not recognize claudin-12 in both samples.

Click here to read more: https://www.scigenus.com/product-page/o-c-t-compound

Sciatic nerve cryosections

Cryosections of the sciatic nerve can be performed with OCT, or optical coherence tomography, by embedding it in 4% PFA for at least 4 hours. The tissue should be mounted such that the bulk of the mass projects above the OCT surface. However, the chuck should be flattened prior to mounting. If the tissue is exposed to a culture solution, it should not be added to it.

For sciatic nerve cryosections using OCT, the lumbar spinal cord must be excised intact and identified. Before beginning serial sectioning, approximately 3 mm of the anterior cord should be removed. After that, the sections should be placed in a cryostat. The chuck with the frozen tissue should be wrapped in parafilm to avoid dehydration. After mounting the frozen tissue, immediately cut the sections. This will enable a better fixation of the sections and minimize the chance of double counting.

The experiments were approved by the Ethics Committee of the Zhejiang Chinese Medical University. Male Sprague-Dawley rats were obtained from the Zhejiang Chinese Medical University Laboratory Animal Research Center. The animals were cared for by keeping the 12-h light/12-h dark conditions and providing ad libitum access to food and water. Animals were anesthetized with 1% pentobarbital sodium.

In some cases, OCT and gelatin may not be compatible. The lower temperature of the latter is necessary to avoid damage to the sample during immunostaining. A gelatin-embedded hard tissue, on the other hand, is more compatible with immunostaining and fluorescent signals. Gelatin-embedded tissues are easier to cryosection and do not require an ultra-sharp blade.

Neurons of the sciatic motor pool, or “sciatal motor neurons,” are found mainly in regions L4-L6. In addition, there are subtle differences between the L1 and L2 regions. The L1 central cord is rounded in shape with a wide U/V, while the L2 central cord is oval in shape and narrower in shape. L2 central cord is also oval in shape with dendrites dispersed throughout the ventral horn.

Another technique that may prove useful is engineered nerve guidance conduits. They were shown to support neuronal regeneration across a large gap. This technique is a useful new approach for fabricating anisotropic engineered tissues. Unlike the use of cadaveric grafts, engineered nerve guidance conduits are easier to produce and have better outcomes than artificially created tissues.

Thanks for visiting wizarticle